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MICROBIOLOGY AND CLINICAL MICROBIOLOGY Course Sheet Academic Year of enrolment:
Disciplinary Sector:
Microbiology and Clinical Microbiology
Professor and Collaborators:
Hours of classroom activity:
Prerequisites:
This course requires that you have taken the course in biology.
Objectives
Contents The course provides learning in the basic principles of medical microbiology and infectious disease. It covers mechanisms of infectious disease transmission, principles of aseptic practice, and the role of the human body’s normal microbiota. The biology of bacterial, viral, and fungal pathogens and the diseases they cause are covered. The course provides the conceptual basis for understanding pathogenic microorganisms and the mechanisms by which they cause disease in the human body. Classes of microorganisms of specific relevance to the common diseases will be discussed in detail.
Extended Syllabus Difference between Prokaryotic and Eukaryotic cells.
Naming and Classifying Microorganisms.
The bacterial cell: Size, shape and arrangements of bacteria.
Structures external to the cell wall: glycocalyx, flagella, pili and fimbriae.
The cell wall: composition and characteristics; cell wall and Gram stain; atypical cell walls.
Structures internal to the cell wall: the plasma membrane; the cytoplasm; the nucleoid; plasmids and resistance factors; ribosomes.
Bacterial metabolism: aerobic respiration; anaerobic respiration and fermentation.
Bacterial division and generation time.
Bacterial spore: structure, types, sporulation and germination, and their protecting role in biocide resistance.
Mechanisms of Bacterial Pathogenesis: entry into the human body; adhesion, colonization and invasion; pathogenic actions of bacteria (tissue destruction, toxins); mechanisms for escaping host defenses. The main bacterial diseases.
Antibacterials and their mechanisms of action. Mechanisms of drug resistance.
Biology of Fungi: general characteristics, structure and classification. Fungal pathogenesis.
Characteristics and classification of viruses.
Virus structure: non-enveloped and enveloped viruses.
Viral replication: life cycle of viruses with animal and prokaryote hosts. The main viral diseases. Antiviral drugs.
Prions.
Hospital-acquired infection.
Disinfection, sterilization and asepsis.
Recommended Bibliography E. Lanciotti: Microbiologia Clinica, CEA - Quarta Ed. 2017.
Murray PR, Rosenthal KS, Pfaller MA: Microbiologia Medica 8a Ed. Edra, 2017.
La Placa: Principi di Microbiologia Medica, EdiSES - Ed. XIV 2014.
Teaching Methods Teaching method: frontal lectures and material provided by the teacher.
Evaluation methods Verification of learning:
Oral examination.
N.B. For Integrated Courses (exams covering more than one module or course unit) a single exam will be held and a final grade given.
Contacts/More Information Contact:
In-Person office hours: Monday – Friday from 10:00 to 13:00.
Or by appointment: Contact the professor by email: nerino.allocati@unich.it .