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Course Sheet Academic Year of enrolment:
Disciplinary Sector:
Microbiology and Clinical Microbiology
Professor and Collaborators:
Hours of classroom activity:
Prerequisites:
This course requires that you have taken the course in biology.
Objectives
Contents The course covers basic principles of general microbiology. Topics include bacterial, viral, and fungal structure, as well as growth, physiology, genetics and biochemistry of microorganisms. The subject covers mechanisms of infectious disease transmission and pathogenicity, principles of aseptic practice, and the role of the human body’s normal microbiota. The course provides learning about microbiological hazards and prevention and protection techniques in the workplace.
Extended Syllabus Difference between Prokaryotic and Eukaryotic cells.
Naming and Classifying Microorganisms.
The bacterial cell: Size, shape and arrangements of bacteria.
Structures external to the cell wall: glycocalyx, flagella, pili and fimbriae.
The cell wall: composition and characteristics; cell wall and Gram stain; atypical cell walls.
Structures internal to the cell wall: the plasma membrane; the cytoplasm; the nucleoid; plasmids and resistance factors; ribosomes.
Bacterial metabolism: aerobic respiration; anaerobic respiration and fermentation.
Bacterial division and generation time.
Bacterial spore: structure, types, sporulation and germination, and their protecting role in biocide resistance.
Mechanisms of Bacterial Pathogenesis: entry into the human body; adhesion, colonization and invasion; pathogenic actions of bacteria (tissue destruction, toxins); mechanisms for escaping host defenses.
Antibacterials and their mechanisms of action. Mechanisms of drug resistance.
Biology of Fungi: general characteristics, structure and classification. Fungal pathogenesis.
Characteristics and classification of viruses.
Virus structure: non-enveloped and enveloped viruses.
Viral replication: life cycle of viruses with animal and prokaryote hosts. Antiviral drugs.
Prions.
Hospital-acquired infection.
Disinfection, sterilization and asepsis.
Microbiological hazards.
Microbiological risk assessment.
Italian decree law: Titolo X D. Lgs 81/08.
Prevention and protection techniques in the workplace.
Hazard analysis and critical control points (HACCP).
Food and water safety and control.
Industrial Microbiology.
Recommended Bibliography E. Lanciotti: Microbiologia Clinica, CEA - Quarta Ed. 2017
La Placa: Principi di Microbiologia Medica, EdiSES - Ed. XIV 2014
Teaching Methods Teaching method: frontal lectures and material provided by the teacher.
Evaluation methods Verification of learning:
Oral examamination.
N.B. For Integrated Courses (exams covering more than one module or course unit) a single exam will be held and a final grade given.
Contacts/More Information In-Person office hours: Monday – Friday from 10:00 to 13:00.
Or by appointment: Contact the professor by email: nerino.allocati@unich.it .