IMMUNOHISTOCHEMISTRY AND MOLECULAR TECHNIQUES

Università degli Studi
"G. d'Annunzio"

Chieti - Pescara

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General Module

Course Sheet

Course code:

TL31C1A

Academic Year of enrolment:

2018

Year of supply:

2020/2021

Type of Course:

Characterising

Degree:

Course of 1st Cycle Degree in BIOMEDICAL LABORATORY TECHNIQUES

Disciplinary Sector:

Laboratory Medicine Techniques

Is part of:

PATHOLOGICAL ANATOMY AND CYTODIAGNOSTIC TECHNIQUES

Location:

CHIETI

Credits:

Programme Year:

Professor and Collaborators:

LATTANZIO ROSSANO

Cycle:

First semester

Obligatory attendance:

Yes

Hours of classroom activity:

Individual study hours:

Prerequisites:

See the prerequisites required by the Course Degree Program

Objectives

The aims of the course are to provide students with the methodological knowledges regarding the use of the immunohistochemical and molecular techniques in clinical practice and research. The acquired informations will allow the student to understand the variety of approaches that can be used to study the tissues and to prepare them to make the most appropriate methodological choices to solve an experimental or diagnostic issue and to critically analyze the experimental results.

Contents

The teaching module aims to provide the student with the methodological bases regarding the ancillary techniques used in a Pathology lab, with particular attention reserved to the immunohistochemical and molecular biology techniques applied to human tissues (in situ hybridization techniques), and to provide the knowledge tools for the correct choice of the technique able to respond to a clinical or research question.

Extended Syllabus

- Principles of the immunohistochemical staining
- How to set up a histological sample for the immunohistochemical staining
- Types of antibodies and methods for the production of monoclonal and polyclonal antibodies
- Physico-chemical properties of the antigen-antibody binding: causes of the antigen unbinding and methods to optimize the use of the immunoreagents
- Antigen identification through immunodetection systems
- Antigen retrieval methods: procedures using heat and protein digestion
- Principal immunohistochemical staining methods
- Pitfalls and technical artifacts in immunohistochemistry
- Application of immunohistochemistry in human pathology
- Tissue Microarray (TMA) sample preparation
- Methods for automatic sample preparation and analysis
- Diagnostic roles of the molecular biology techniques (i.e., FISH, CISH and RNAscope methods) in human pathology
- Use of the cDNA and RNA microarray technology for the study and characterization of tumors

Recommended Bibliography

The recommended texts are listed below:
- Bancroft "Theory and Practice of Histological Techniques", 8th Edition, Elsevier (2018)
- Pranab Dey "Basic and Advanced Laboratory Techniques in Histopathology and Cytology", Springer (2018)
- Luigi Ruco, Aldo Scarpa "Anatomia Patologica, Le Basi", UTET, Scienze Mediche (2007)
The teaching slides and the related scientific publications (i.e., “open-access” publications) will be made available for the student after the lessons.

Teaching Methods

Lessons by frontal teaching

Evaluation methods

Verification of learning:

The course will be held through frontal lessons. The student preparation will be tested through oral questions with the final exam score expressed in thirtieths. The student must have learned the general principles of the immunohistochemical staining methods applied to the human tissues, and of the main molecular biology techniques.

Contacts/More Information

The reception of the students will be provided by the teacher upon appointment to be requested via “Teams” platform or via the following e-mail address: rossano.lattanzio@unich.it

Università degli Studi
"G. d'Annunzio"

Chieti - Pescara

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