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HISTOPATHOLOGY TECHNIQUES Course Sheet Academic Year of enrolment:
Disciplinary Sector:
Laboratory Medicine Techniques
Professor and Collaborators:
Hours of classroom activity:
Prerequisites:
See the prerequisites required by the Course Degree Program
Objectives
Contents The teaching module aims to provide the student with the basic knowledge about the performed activities of a pathology lab, and to elucidate the main procedures for fixing, processing and staining of the histological samples
Extended Syllabus - Basics of Optical Microscopy
- Procedures for acceptance of the biopsy, and macroscopic examination of the surgical samples, with particular attention reserved to the extemporaneous examination
- General principles of the main techniques of fixation, of tissue processing, of the embedding procedures and sectioning of the paraffin-embedded blocks
- Sectioning and staining protocols according to the international guidelines
- Staining methods: particular attention will be reserved to the hematoxylin and eosin and some of the frequently used histomorphological, histochemical and special staining techniques
- Tissue processing: pitfalls and technical artifacts
Recommended Bibliography The recommended texts are listed below:
- Bancroft "Theory and Practice of Histological Techniques", 8th Edition, Elsevier (2018)
- Pranab Dey "Basic and Advanced Laboratory Techniques in Histopathology and Cytology", Springer (2018)
- Luigi Ruco, Aldo Scarpa "Anatomia Patologica, Le Basi", UTET, Scienze Mediche (2007)
The teaching slides and the related scientific publications (i.e., “open-access” publications) will be made available for the student after the lessons.
Teaching Methods Lessons provided in the classroom by frontal teaching
Evaluation methods Verification of learning:
The course will be held through frontal lessons. The student preparation will be tested through oral questions with the final exam score expressed in thirtieths. The student must have learned the general principles of the histological methods used in a Pathology Lab
Contacts/More Information The reception of the students will be provided by the teacher upon appointment to be requested online by “Teams” platform or via the following e-mail address: rossano.lattanzio@unich.it