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DIAGNOSTIC LABORATORY (SECOND SEMESTER I) Course Sheet Academic Year of enrolment:
Professor and Collaborators:
Hours of classroom activity:
Objectives
Contents The internship is proposed:
a) educating the student to the standards of hardness in a laboratory
b) to provide students with the basics of use in the molecular biological / biotechnological field
Extended Syllabus Safety standards in the laboratory
-preparation of buffer solutions
- cell cultures
- the hood
- work in sterility
- cell count with Burker chamber
- incubator-preparation of agarose and acrylamide gels
- cellular homogenates
- PCR
-Western blot
Recommended Bibliography
Teaching Methods Frequency with practical application of the study in the biology laboratory, directed by the teacher
Evaluation methods Verification of learning:
The valuationent will be attributed with a student's suitability judgment based on the ability of the student to work in safety, demonstrating that he understood the rationale of each topic treated in the laboratory and how to act to make the topics acquired practical.
Contacts/More Information Prof.ssa Sara Franceschelli: Università degli studi G. D'Annunzio, Palazzina Farmacia corpo D livello 1
Tel. 0871-3554539 email: sara.franceschelli@unich.it