During the internship, the student will acquire the basic techniques for Microbial growth and biofilm development by Gram-positive and Gram-negative bacteria. The student will be made aware of the current safety precautions of the laboratory as well as the use of individual and collective security devices.
The student will learn:
- to prepare the culture media;
- to grow bacteria;
- to develop a Biofilm;
- the Live/Dead staining for the sample visualization by using CLSM;
- to analyze and count the Colony Forming Units (CFUs);
- to isolate and characterize the bacterial vesicles.
The instrument used will be:
- the autoclave;
- laminar flow hood;
- incubator;
- heating plates;
- spectrophotometer;
- centrifuges at low and high speeds (ultracentrifuge);
- microscope;
- ELISA reader;
- PCR.