FORENSIC ANALYTICAL CHEMISTRY

Università degli Studi
"G. d'Annunzio"

Chieti - Pescara

Single discipline educational activity

Course Sheet

Course code:

CAF010

Academic Year of enrolment:

2018

Year of supply:

2021/2022

Type of Course:

Elective Course

Degree:

Course of Single Cycle Degree 5 years in MEDICINAL CHEMISTRY AND PHARMACEUTICAL TECHNOLOGY

Disciplinary Sector:

Analytical Chemistry

Language:

Italian

Location:

CHIETI

Credits:

Programme Year:

Professor and Collaborators:

CARLUCCI Giuseppe

Cycle:

Second semester

Obligatory attendance:

Yes

Minimum hours of attendance:

Hours of classroom activity:

Individual study hours:

Prerequisites:

Having taken the exam in General and Inorganic Chemistry, and Analytical Chemistry

Objectives

Knowing the information obtainable from chemical analysis - Knowing the potential and limits of chemical analysis in the forensic field - Designing a chemical analysis to be able to discuss it in court.

Contents

Classification of the methods of analysis in relation to the problems and expected results. The problem of sampling in forensic science, significance and specificity of the sample with respect to the environment. The function of the "blank test". The blood and urine. Doping, drugs of abuse. Procedures for qualitative and quantitative analysis in relation to the different scenarios. Interpretation of the results: the margin of uncertainty of the analytical results and the importance of the approach with different analytical techniques according to the formation of the "test". Sensitivity and limit of detection in forensic use. The analysis execution strategies based on the accuracy of the analysis. Criteria for evaluating the significance of results as a function of their use in forensic science. Instrumental methods and micro-extraction techniques in sample preparation.

Extended Syllabus

Introduction to the course, the role of the forensic analytical chemist. The sample and its collection, manipulation for analysis. The biological matrices: blood, plasma, saliva, serum and urine. Unconventional matrices: hair, nails, hair, sweat. Solid and liquid phase micro-extraction techniques such as solid-phase micro-extraction (SPME), packed adsorbent micro-extraction (MEPS), solid-phase extraction (SPE) liquid dispersive liquid micro-extraction (DLLME), dried blood spot (DBS). Enzymatic treatment of urine samples for the release of analytes. Spot-test for the detection of blood traces on different surfaces through the use of Luminol. Equipment necessary for their detection. Dry blood drop analysis through online and offline DBS. The role of hematocrit in sampling with DBS. Seminal plasma: methods of recognition of biological material on different matrices. Doping: analysis techniques of the main analytes reported by WADA. Masking and screenenin techniques, confirmation techniques for the main doping agents. Drugs of abuse and their determination. The cathinones. The impressions: typology and detection on different surfaces with reactive details. The inks. The technical consultant in the processes. The compilation of reports in the sampling phase. Food fraud. Optical analysis techniques (spectrophotometric and fluorimetric). Separation techniques, high efficiency liquid chromatography (HPLC) and ultra high efficiency (UHPLC) single and coupled to mass spectrometry (UHPLC-MS).

Recommended Bibliography

• G. Carlucci – Cromatografia Liquida ad Alta Efficienza HPLC- Applicazione all’analisi di farmaci in matrici biologiche – Libreria Benedetti L’Aquila
• Lecture notes.

Methods of Provision

Conventional

Teaching Methods

The teaching will be divided into lectures through the use of transparencies and Power Point, laboratory experiences and classroom exercises during class time.

Evaluation methods

Verification of learning:

Verification of learning will be carried out through an oral exam.

Contacts/More Information

The textbooks are recommended, the student is not required to buy them all, but the topics covered are widely discussed in the titles shown. The teacher is available during office hours for further clarifications on the topics covered in class.

Università degli Studi
"G. d'Annunzio"

Chieti - Pescara

Aree