Frontal lessons
The origin of the life, the role of microorganisms in the biosphere.
Structure and functions of bacterial cell: cytoplasm, DNA, cytoplasmic membrane and cell wall. Optional structures (capsule, pili and flagella).
Biological risk. Biologica agents classification. Microrganisms and environment. DPI.
The microbial growth, the growth curves, the bacterial division. Microbial metabolisms.
Techniques for in vitro microbial cultures. The biological risk.
Survival strategies of bacteria: spore production, L-forms, viable but not cultivable state, biofilm.
Microbial pathogenicity and epidemiology: host-microrganism relationships, pathogenic properties of bacteria.
Human microbiota. Probiotics and prebiotics.
Nosocomial infections, opportunistic human pathogens. Index of Microbial Contamination, IMC.
Gene recombination in bacteria: transformation, conjugation, transduction. Lysogenic conversion.
Sterilization. Disinfectants, antiseptics and preservatives. Sterilization of pharmaceutical products. Good practices in medicinal preparation.
The Microbiology in the cosmetic industry. The microbiological approach in the cosmetic field.
Antimicrobial agents. Clinical use of antimicrobial drugs. Mechanisms of action of antibiotics. Bacterial resistance to antibiotics. One Health approach. Methods for testing of antibiotics.
Microbiological tests and dosages of the Pharmacopoeia: Evaluation of pharmaceutical microbial contamination. Biological tests.
Structure and classification of viruses. Viral multiplication. Virus-host cell interactions. Bacteriophages.
Structure, habitat and reproduction of molds and yeasts.
Microbiology lab Practice
Sterilization techniques, Preparation of two-fould dilutions of broth-cultures and speading on microbial media. Count and analysis of colony-forming units. Broth-culture preparation. Slide preparation and staining, microscopic observation by optical microscope. Antimicrobial susceptibility tests
