-Introduction
In vivo and in vivo studies
-Buffers and Medium
Bacterial medium
-Mammalian cell culture medium
Cell Culture
Primary cells
Immortalized cell lines
Storing cell lines
-Molecular Biology
DNA and RNA: introduction
DNA and RNA purification
Agarose gel electrophoresis
Restriction Enzymes
Ligase
Cloning vectors, Expression vectors
DNA sequence analysis
cDNA library and gDNA library
Retrotrascription
PCR and Real Time PCR
Mutagenesis, Transgenic and KO animal models
Mammalian cell transfection methods
Promoter assay
iRNA
-Proteins
proteins purification and characterization
-Electrophoresis
PAGE gels
Agarose gels
-Protein-protein interaction assays
Two Hybrid system
Pull-Down
Duo-Link
-DNA-protein interaction assays
EMSA
ChIP
-Microarray
-Proteomics techniques
-Bioinformatic analysis
sequence alignment and sequence database