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MOLECULAR BIOTECHNOLOGIES AND GMOs Single discipline educational activity
Course Sheet Academic Year of enrolment:
Professor and Collaborators:
Minimum hours of attendance:
Hours of classroom activity:
Prerequisites:
To take the Molecular Biotechnology and GMOs exam you must have passed the Biochemistry exam
Objectives Contents Structure of nucleic acids, organisation and maintenance of genomes, transcription and translation, regulation of gene expression. Main genome editing techniques. Use of GMOs in the pharmaceutical industry for the production of recombinant proteins, in agricultural production and breeding to improve production and resistance to pathogens. Genetic and epigenetic damage induced by mutagenic agents and techniques for their monitoring. Use of microorganisms for waste treatment and/or remediation of contaminated environments.
Extended Syllabus General principles- Structure of nucleic acids, organisation of eukaryotic and prokaryotic genomes, genome replication and maintenance, transcription, mRNA maturation, translation, epigenetic regulation, non-coding RNA.
Environmentally induced genetic alterations- DNA repair systems, epigenetic modifications, gene and chromosome mutations.
Technologies- Gene expression study, PCR, cloning, sequencing methods, in vitro and in vivo gene toxicity testing.
Genetically modified organisms- Modification of eukaryotic and prokaryotic cells: methods of over-expression and gene silencing, gene editing.
Use of genetically modified organisms for waste disposal and/or valorisation.
Genetically modified animals: methods for over-expression, gene silencing and gene editing; use of genetically modified animals in scientific research and in the pharmaceutical industry and associated risks (biosafety).
Genetically modified plants: methods for over-expression, silencing and gene editing; use of genetically modified plants in agriculture (herbicide and insect resistance, quality and nutritional improvements) and industry (plants as bioreactors) and associated risks (biosafety).
Recommended Bibliography BIOLOGIA MOLECOLARE; Casa Editrice Ambrosiana
FONDAMENTI DI BIOLOGIA MOLECOLARE
BIOTECNOLOGIE MOLECOLARI
Methods of Provision Teaching Methods Teaching will be delivered mainly through lectures with slides and explanations. Some concepts will be consolidated through laboratory experiences
Evaluation methods Verification of learning:
The assessment of learning will be carried out at the end of the course, at the pre-established appointments, by means of an oral test covering several topics.
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